Abbexa cdan1 siRNA instruction manual

Use  RNA  Interference suppression  CDAN1  Interacting nuclease  1 (CDIN1)  Express  siRNA. AI Meijie Abbexa CDAN1 siRNA  With three  5 nmol  Little bottle  (15 nmol)  or  2x  Three  5 nmol  Little bottle  (30 nmol)  Freeze drying  siRNA  The form of oligonucleotide duplexes provides . Each vial contains a slightly different sequence , To ensure complete gene knockout . Double stranded bodies can be transfected alone or pooled together , To achieve target gene knockdown , This is usually done through  qPCR  Or Western blot .

AI Meijie Abbexa CDAN1 siRNA The basic parameters ：

The goal is  CDAN1- Interacting nuclease  1 (CDIN1)

Reactivity   human beings

The test application   RNA interference

Recommended dilution   Accurate dilution / The concentration shall be determined by the user .

form   Freeze drying

The purity  > 97%

The quality control   Base by base monitoring of oligonucleotide synthesis by triphenylmethyl analysis , To ensure proper coupling efficiency . Subsequently, oligonucleotides were purified by affinity solid phase extraction . Further analysis of annealed by mass spectrometry  RNA  Double chain , To verify the exact composition of the double chain . Compare each batch with the previous batch by mass spectrometry , To ensure maximum inter batch consistency .

Keep in storage   stay  4 °C  Lower transportation . stay  -20 °C  Store for up to a year .

UniProt  Primary alternating current  Q9Y2V0 ( UniProt , ExPASy )

Gene symbol  C15orf41

gene ID 84529

Specificity  C15orf41 siRNA (Human)  It is a target specificity  19-23 nt siRNA  Oligonucleotide double stranded body , To reduce gene expression .

AI Meijie Abbexa CDAN1 siRNA Instructions ：

1、 Before overhanging , Centrifuge the tubes briefly to ensure that they are freeze-dried  siRNA  At the bottom of the pipe .

2、 use  DEPC  Water will  siRNA oligo  Resuspend to appropriate concentration （ for example , Put a bottle  5 nmol siRNA oligo  Hang heavy on  250 µl DEPC  in the water , The final concentration is  20 µM）.

3、 Before cell lysis  48  to  72  For hours  10 nM - 100 nM siRNA  transfection .

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stay ：GeneReviews [ Internet ]. Seattle （WA）： University of Washington , Seattle ;1993-2022  year .

2009  year  4  month  21  Japan [ to update 2021  year  7  month  29  Japan ] .

Affiliation   expansion

PMID: 20301759  Bookshelf number ： NBK5313