Lefse analysis

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LEfSe（LDA Effect Size） analysis , It can be used for comparison between two or more groups , So as to find the Group Species with significant differences （ namely biomarker）, The analysis steps are mainly divided into three steps ：

Step1： utilize Kruskal-Wallis Rank sum test detects all characteristic species , By detecting species abundance between different groups Degree difference , Obtain significantly different species .

Step2： recycling Wilcoxon Rank sum test tests whether all subspecies of the species with significant differences obtained in the previous step tend to The same classification level .

Step3： Finally, linear discriminant analysis （LDA）, Get the final differential species （ namely biomarker）.

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