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lefse分析
2022-07-01 18:35:00 【全栈程序员站长】
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LEfSe(LDA Effect Size)分析,可以用于两个或多个分组之间的比较,从而找到组间 有显著性差异的物种(即 biomarker),分析步骤主要分为三步:
Step1:利用 Kruskal-Wallis 秩和检验检测所有的特征物种,通过检测不同组间的物种丰 度差异,获得显著性差异物种。
Step2:再利用 Wilcoxon 秩和检验检测上步获得的显著性差异物种的所有亚种是否都趋 同于同一分类级别。
Step3:最后用线性判别分析(LDA),得到最终的差异物种(即 biomarker)。
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