Characteristics and determination scheme of Worthington pectinase

AI Meijie Worthington  Pepsin is an acid protease . Its inactive proenzyme pepsinogen is produced in the gastric mucosa . Several pepsins are named  A、B、C  and  D. Pepsin  A  Is the main ingredient , Its molecular weight is  35,000  Dalton , If the substrate is natural protein , The optimum substrate such as casein or hemoglobin  pH  about  1.0. Pepsin is preferentially in aromatic amino acids （ Such as phenylalanine and tyrosine ） Cleave the protein at the carboxyl group of . It will not contain valine 、 The bond of alanine or glycine breaks . be based on  Anson J. Gen. Physiol., 22 , 79 (1938)  The method uses hemoglobin as a substrate to determine pepsin . Pepsin in  pH  The value is higher than  6  Unstable when .

stability / Store ： Pepsin in  2-8°C  Save  1-2  year .

Unit definition ： A unit in  37°C  Release from denatured hemoglobin every minute  0.001 A280  As  TCA  Soluble hydrolysates . One  FIP  Company , With  25°C  The equivalent micro moles of tyrosine released per minute under , It can be calculated as follows ：1  Worthington unit  x 0.0071 = FIP  Company .

   

 

Worthington The stomach Application of protease ：

Digestion of antibodies

Preparation of collagen for cosmetic purposes

Evaluation of protein digestibility in Food Chemistry

Subculture of living mammary epithelial cells  (Riser 1983)

AI Meijie Worthington Pepsin Related research ：

Collagenase  (CLS1-4/CLSPA)

Deoxyribonuclease  I (DP/D/DCLS/D2/DPFF/DPRF)

Hemoglobin  (HB)

Hyaluronidase  (HSE/HSEP)

Neutral protease  (Dispase, NPRO)

protease  K (PROK)

Related literature ：

1.Absolon, K.： Use albumin  I 131 (RIHSA)  Determine proteolytic and anti proteolytic activities , Surg  Forum  5 , 543, 1954

2.Al -Janabi, J.、Hartsuck, J.  and  Tang, J.： Kinetics and mechanism of pepsinogen activation , J Biol Chem 247 , 4628, 1972

3.Anderson, W.  and  Harthill, J.： Pepsin inhibitory activity in pepsinogen activating peptide , Nature 243 , 417, 1973