BioVendor Human Club Cellular Protein (CC16) Elisa Kit Research Fields

The human club cell proteins (CC16, CC10, uteroglobin, urinary protein 1, or Clara cell secreted proteins) are members of the secretoglobin family and are secreted by non-ciliated bronchiolar club cells.Its function remains to be fully elucidated, but there are compelling data for its role as an immunomodulatory and anti-inflammatory agent.Club cell protein inhibits phospholipase A2 activity as well as interferon gamma signaling and Th1 and Th2 lymphocyte regulation.

Club cell protein concentrations in serum, plasma and bronchoalveolar lavage fluid have been determined in numerous studies since 1994.In serum, its increase was associated with age and exposure to asbestos, nitrogen chloride and ozone.Higher levels of CC16 were demonstrated in patients with sarcoidosis, pulmonary fibrosis and high PEEP ventilation.Reduced serum CC16 levels were found after lung resection in smokers and subjects with chronic obstructive pulmonary disease, asthma, or silica exposure.

Reduced CC16 concentrations were also found in the amniotic fluid of fetuses with pulmonary hypoplasia caused by various mechanisms (diaphragmatic hernia, diabetic fetal disease, Turner and Down syndrome).In pleural effusions, CC16 concentrations appear to correlate with its diffusion from the lungs, as evidenced by high CC16 levels in cardiac pleural congestion.Based on the above reports, club cell proteins may be useful diagnostic markers for lung disease and injury.

Ai Meiji BioVendor Human Club Cell Protein (CC16) Elisa Kit Chemical Properties:

Type: Sandwich ELISA, Biotinylated Antibody

Other names: CC10, CC16, uteroglobin, urine protein 1, Clara cell secreted protein, casein kinase 1, CK1

Species: Human

Application: Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, COVID 19

Sample requirement: 5 μl/well

Storage/Expiration: Store the kit at 2–8°C.Under these conditions, the kit is stable until the expiration date (see label on box).

Calibrated range: 1.57–50 ng/ml

Detection limit: 46 pg/ml

Within assay (within run): n = 8; CV = 3.4%

Run-to-Run: n = 4; CV = 4.7%

Research topics: immune response, infection and inflammation, lung disease, COVID 19

AmigenBioVendorHuman Club Cell Protein (CC16) Elisa KitFeatures:

EU: For in vitro diagnostics

Other Regions: Research Use Only!

Total testing time is less than 4 hours

The kit can measure total club cell proteins (EDTA, citrate, heparin) in serum and plasma

The detection format is 96 wells

Quality control based on human serum

Standard is based on recombinant protein (E. coli)

The components of the kit can be used directly, concentrated or lyophilized

BioVendorHuman Club Cell Protein (CC16) Elisa KitClinical Uses and Research Fields:

Pneumonia and bronchopneumonia

chronic obstructive pulmonary disease, sarcoidosis, pulmonary fibrosis

Acute lung injury

Asthma and allergic rhinitis

Lung cancer

IgA nephropathy

Literature references:

1.Alexis NE, Lay JC, Haczku A, Gong H, Linn W, Hazucha MJ, Harris B, Tal-Singer R, Peden DB. Fluticasone propionate protects against ozone-induced airwayinflammation and modified immune cell activation markers in healthyvolunteers.Environ Health Perspect. 2008 Jun;116(6):799-805. doi: 10.1289/ehp.10981. PubMed PMID: 18560537; PubMed Central PMCID: PMC2430237. See more on PubMed

2.Andersson L, Lundberg PA, Barregard L. Methodological aspects on measurement of Clara cell protein in urine as a biomarker for airway toxicity, compared with serum levels. J Appl Toxicol. 2007 Jan-Feb;27(1):60-6. PubMed PMID: 17186574. See more on PubMed

3. Aul R, Armstrong J, Duvoix A, Lomas D, Hayes B, Miller BE, Jagger C, Singh D. Inhaled LPS challenges in smokers: a study of pulmonary and systemic effects. Br J Clin Pharmacol. 2012 Dec;74(6):1023-32. doi: 10.1111/j.1365-2125.2012.04287.x. PubMed PMID: 22469312; PubMed Central PMCID: PMC3522816. See more on PubMed