Lumiprobe proteorange protein gel dye instructions

AI Meijie Lumiprobe ProteOrange  Protein gel dye background ：

ProteOrange  It is a protein binding dye for protein gel dyeing , yes  SYPRO Orange  Analogues . Fluorophores are amphoteric dyes similar to styrene . It is in the presence of nucleic acids 、 In the case of polysaccharides and other molecules, they selectively bind to proteins . It also tolerates  SDS, So it can be used in  SDS-PAGE  Gel dyeing . The dye interacts with the surrounding proteins in the gel  SDS  Coating interaction , Therefore,  CBB  Dyeing compared to , It can provide more consistent staining between different types of proteins , And no negative staining . It can also stain glycoproteins very well .

ProteOrange  Dyeing ratio  Coumassie  Dye dyeing sensitivity is about an order of magnitude , Each band can detect about  3 ng  Protein （Coumassie  The detection limit of is  30 ng）.

ProteOrange  With  DMSO  Medium  5000x  In solution form .1 mL  The solution is sufficient to stain about  200  A little gel .

AI Meijie  Lumiprobe ProteOrange  With protein and  SDS  Excitation and emission spectra of the complex ：

AI Meijie Lumiprobe ProteOrange  Protein gel dye Related research ：

Cyanine5 NHS  Ester is the smallest dye

Cy5 NHS  Ester minimum dyes are used for protein labeling .

Cyanine3 NHS  Ester is the smallest dye

For protein labeling  Cyanine3  The smallest dye .

Cyanine2 NHS  Ester is the smallest dye

For protein labeling  Cyanine2 NHS  Ester is the smallest dye

3Dye 2D DIGE  Kit

Lumiprobe 3Dye 2D DIGE  Kit yes  GE Healthcare  A substitute for the dye produced .

For two-dimensional differential gel electrophoresis  3Dye Minimal Labeling Kit

For differential analysis of proteome samples  2D DIGE（ Two dimensional gel electrophoresis ） Kit .Lumiprobe 3Dye 2D DIGE Kit  yes  GE Healthcare  A substitute for the dye produced .