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Lumiprobe ProteOrange 蛋白质凝胶染料说明书
2022-06-28 18:01:00 【Sylvia_sc】
艾美捷Lumiprobe ProteOrange 蛋白质凝胶染料背景:
ProteOrange 是一种用于蛋白质凝胶染色的蛋白质结合染料,是 SYPRO Orange 的类似物。荧光团是一种类似苯乙烯的两性离子染料。它在存在核酸、多糖和其他分子的情况下选择性地结合蛋白质。它还耐受 SDS,因此可用于 SDS-PAGE 凝胶染色。该染料与凝胶中蛋白质周围的 SDS 涂层相互作用,因此与 CBB 染色相比,它可以在不同类型的蛋白质之间提供更一致的染色,并且不会出现负染色。它还可以很好地染色糖蛋白。
ProteOrange 染色比 Coumassie 染料染色灵敏约一个数量级,每条带可检测约 3 ng 的蛋白质(Coumassie 的检测限为 30 ng)。
ProteOrange 以 DMSO 中的 5000x 溶液形式提供。1 mL 溶液足以染色约 200 个小凝胶。
艾美捷 Lumiprobe ProteOrange 与蛋白质和 SDS 复合物的激发和发射光谱:

艾美捷Lumiprobe ProteOrange 蛋白质凝胶染料相关研究:
Cyanine5 NHS 酯最丨小染料
Cy5 NHS 酯最丨小染料用于蛋白质标记。
Cyanine3 NHS 酯最丨小染料
用于蛋白质标记的 Cyanine3 最丨小染料。
Cyanine2 NHS 酯最丨小染料
用于蛋白质标记的 Cyanine2 NHS 酯最丨小染料
3Dye 2D DIGE 套件
Lumiprobe 3Dye 2D DIGE 试剂盒是 GE Healthcare 生产的染料的替代品。
用于二维差异凝胶电泳的 3Dye Minimal Labeling Kit
用于蛋白质组样本差异分析的 2D DIGE(二维凝胶电泳)试剂盒。Lumiprobe 3Dye 2D DIGE Kit 是 GE Healthcare 生产的染料的替代品。
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