Chinese and English instructions - human alpha fetoprotein (AFP) ELISA quantitative Kit

Specification：

Product name：EliKine Human Alpha Fetoprotein (AFP) ELISA Kit

Reactivity：Human

Applications：ELISA

Applications notes：EliKine Human Alpha Fetoprotein (AFP) ELISA Kit employs a double antibody sandwich method to quantitate Human AFP in samples. An antibody specific for Human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human AFP present is bound by the immobilized antibody. After removing any unbound substances, an HRP-conjugated antibody specific for Human AFP is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Alpha Fetoprotein (AFP) concentration is proportional to the OD450 nm value.

Detection method Colorimetric

SampleType：Cell culture supernatants, Plasma, Serum

Assay type：Sandwich ELISA (quantitative)

Assay duration：Multiple steps standard sandwich ELISA assay with a working time of 3.5 hours. It depends on the experience of the operation person.

Product Properties：

Kit components：

• Human AFP Microplate

• Human AFP Standard (lyophilized)

• Sample Diluent (5×)

• Assay Buffer (5×)

• HRP-conjugated Human AFP Detect Antibody (100×)

• HRP Substrate（TMB）

• Stop Solution

• Wash Buffer (20×)

• Plate Covers

Features & Benefits：EliKine Human Alpha Fetoprotein (AFP) ELISA Kit has high sensitivity and excellent specificity for detection of Human Alpha Fetoprotein (AFP), No significant cross-reactivity or interference between Human Alpha Fetoprotein (AFP) and analogues was observed.

Calibration range：0.625-40 ng/mL

Limit of detection：0.313 ng/mL

Abbkine Human alpha fetoprotein （AFP）ELISA Quantitative kit Experimental suggestions ：

EliKine Human alpha fetoprotein （AFP）ELISA The quantitative kit uses double antibody sandwich method to quantify human alpha fetoprotein in the sample （AFP）. Anti human alpha fetoprotein （AFP） The specific antibody is coated on the enzyme label plate , Add the sample or standard into the hole of the enzyme label plate , Human alpha fetoprotein （AFP） It will bind to the solid-phase antibody coated on the plate . Unbound ingredients are washed away , And then HRP Coupled anti human alpha fetoprotein （AFP） Specific antibody is added into the hole . Remove unbound HRP  After antibody coupling , Add chromogenic substrate （TMB）,TMB  stay  HRP  Under catalysis, it appears blue , It turns yellow after adding termination liquid . Use a microplate reader in  450 nm  Wavelength measurement OD  value , Human alpha fetoprotein （AFP） Concentration and  OD450  Values are proportional .

EliKine Human alpha fetoprotein （AFP）ELISA Advantages of quantitative kit ：

Its detection has high sensitivity and excellent specificity , No association with human alpha fetoprotein was observed （AFP） Significant cross reactions or interferences between analogues .

Precautions in use ：

1.  If  Sample Diluent (5×) and  Assay Buffer (5×) The color turns yellow , A small amount of precipitation and other phenomena , It is because the reagent contains serum , Centrifuge to remove the sediment , Does not affect normal use .

2.  Different batch numbers 、 Do not mix components from different manufacturers ; otherwise , May cause abnormal results .

3.  Change suction head frequently , Avoid cross contamination between components .

4.  To ensure accurate results , The plate must be adhered correctly during the incubation step .

5. Stop Solution  It is corrosive , Please take protective measures during operation .