Characteristics and experimental suggestions of abbkine abfluor 488 cell apoptosis detection kit

Apoptosis （apoptosis） It is a basic biological phenomenon , It refers to maintaining the stability of the internal environment , Cells controlled by genes die spontaneously and orderly , It plays a necessary role in removing unwanted or abnormal cells in multicellular organisms . In normal living cells , Phosphatidylserine （PS） Located on the inner side of the cell membrane , In the early stage of apoptosis ,PS It can be turned from the inner side of the cell membrane to the surface of the cell membrane , Exposure to the extracellular environment . Annexin V(Annexin V)  It is a kind of with molecular weight of 35~36KDa Calcium dependent phospholipid binding protein , Can and PS High affinity and strength specific combination . Labeled with fluorescein or biotin Annexin V It can simply and quickly detect the early apoptosis of cells . Propidium iodide （propidine iodide,PI） It is a nucleic acid dye , It cannot penetrate the intact cell membrane , The cell membrane of early apoptotic cells is intact , Yes PI Have dye repellency . But in the middle and late stages of apoptosis, cells and dead cells ,PI It can penetrate the cell membrane and stain the nucleus . So it will Annexin V  And PI  Match to use , You can distinguish between cells in the early and late stages of apoptosis and dead cells .PI Can be 488,532 or 546nm Laser line excitation , And emit red fluorescence .

Experimental suggestions ：

Abbkine Annexin V-AbFluor 488  Apoptosis detection kit （ Green fluorescence ） contain Abbkine Proprietary AbFluor 488  Marked with green fluorescent dye Annexin V, Apoptotic cells can be identified and quantified by flow cytometry or fluorescence microscope .

AbFluor 488  Apoptosis detection kit Component ：

• Annexin V  Binding buffer (5x)

• Annexin V- AbFluor 488

• Propidium Iodide (PI)

AbFluor 488  Apoptosis detection kit advantage ：

• Abbkine AbFluor 488  yes FITC A good substitute for , Not only the fluorescence intensity and photostability are better , And don't suffer pH Influence

• Annexin V- AbFluor 488: Ex/Em = 491/517 nm; PI: Ex/Em = 535/617 nm (DNA dyestuff )

• Suitable for flow cytometry and fluorescence microscopy

AbFluor 488  Apoptosis detection kit Example show ：

chart ： Camptothecin induced Hela cells 24 Hours ,Annexin V-AbFluor dyeing  488 Apoptosis detection kit . Cells have annexins V-AbFluor Late apoptosis / Necrotic cells  488 and PI dyeing （ Green film , Red debris core ）.