Lumiprobe cell biology - dia, instructions for lipophilic tracer

AI Meijie Lumiprobe DiA, Lipophilic tracer background ：

DiA  It is a dialkyl aminostyryl dye , When it is inserted into the membrane or diluted into an organic solvent, it will emit fluorescence .DiA  The maximum excitation wavelength of is  460 nm, The maximum emission wavelength is  580 nm. Because of its wide emission spectrum ,DiA  Can be in green 、 Detected in orange and red channels , It depends on the filter used .

DiA  It is widely used as anterograde and retrograde neuronal tracers in living and fixed tissues and cells .DiA  Neurons are uniformly labeled by diffusion in the plasma membrane . In the whole organization , Dye does not transfer from labeled cells to unlabeled cells , But when the membrane is destroyed , For example, after slicing , There may be some shifts .

DiA  It can be used with other neuronal tracers （ for example  DiI） Used together as a second color neuron tracer .

 

AI Meijie  Lumiprobe DiA, Lipophilic tracer chemical properties ：

Molecular weight ： 787.06

Molecular formula ： C 46 H 79 Into the 2

The quality control ：NMR 1 H  and  HPLC-MS (95+%)

Storage conditions ： stay  -20°C  In the dark  24  Months . transport ： At room temperature for  3  Zhou . dry .

AI Meijie  Lumiprobe  DiA, Lipophilic tracer Related research ：

BDP 493/503  Lipid stain

BDP 493/503  It is a lipophilic boron Dipyrrole methylene dye , For staining and tracing lipids and oils .

BDP 505/515  Lipid stain

BDP 505/515  It is a kind of lipid dye for cell membrane . It has high fluorescence quantum yield , And very light resistant .