Nanoprobes Polyhistidine (His-) Tag: Recombinant Protein Detection Protocol

Ai Meiji Nanoprobes GoldiBlot: View polyhistidine-tagged proteins in about 1 hour, Ni-NTA-Nanogold binds directly to His tags, no antibodies or equipment required!

Nanoprobes Five GoldiBlot Features:

1. Fast - results in about 1 hour!

Ni-NTA-Nanogold binds directly to the His tag

No more time-consuming antibody incubations!

2. Colorimetric detection - no film, no equipment required!

Dark red, permanent signal, low background, directly visible to the naked eye

3. Highly sensitive - as low as 1 ng!

4. Province Province

No more expensive antibodies!

About $10 per blot

5. Load CRUDE or purified His-tagged protein

*Nanogold, FluoroNanogold, Undecyl Gold, or Colloidal Gold can also be custom conjugated to primary antibodies, peptides, small molecules, or other molecules.

Ai Meiji NanoprobesPolyhistidine (His-) tags for other studies:

Ni-NTA-Nanogold:

2080-3ML 1.8 nm Ni-NTA-Nanogold

2082-3ML 5 nm Ni-NTA-Nanogold

2084-3ML 10 nm Ni-NTA-Nanogold