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Abnova membrane protein lipoprotein technology and category display
2022-07-07 07:08:00 【Sylvia_ sc】
In vitro wheat embryo system + Lipoprotein Technology
AI Meijie Abnova The in vitro protein expression system was developed based on the eukaryotic translation mechanism of wheat embryo . The wheat germ stores all the components needed for translation in a dry and concentrated state , Protein synthesis occurs at the beginning of the embryo . Common wheat The embryo extract contains RNA N- Glucosidase malt agglutinin and other translation inhibitors , Such as Thionine 、 Ribonuclease 、 Deoxyribonucleases and proteases, etc . These inhibitors are produced in the endosperm . Remove endosperm from wheat embryos by washing them thoroughly Contaminants , Form highly stable and active extracts .
Will have 5' Terminal methylation and 3' End poly(A) Tail mRNA React with this extract , At the same time, the unique liposome was added , The translation reaction in vitro can produce enough membrane proteins , Membrane proteins are captured by liposomes , So as to correctly fold out the protein forms necessary for the implementation of biological functions . Common protein expression systems , E. coli, for example 、 Insect cells and mammalian cells belong to the body system and are bound by the cell membrane , Its yield and stability are relatively low . Compared with these protein expression systems , AI Meijie Abnova This system has remarkable advantages . Besides , The in vitro wheat embryo system can stand the test of automated high-throughput small molecule biological screening and characterization .
characteristic :
Higher production
More stable
High expression success rate
Natural protein configuration
Easy to use for automation
Category :
Cluster differentiation (CD) molecular
G Protein coupled receptors (GPCR)
ion channel
Transporters
AI Meijie Abnova Membrane protein lipoprotein demonstration :
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