Abnova membrane protein lipoprotein technology and category display

In vitro wheat embryo system  +  Lipoprotein Technology

AI Meijie  Abnova The in vitro protein expression system was developed based on the eukaryotic translation mechanism of wheat embryo . The wheat germ stores all the components needed for translation in a dry and concentrated state , Protein synthesis occurs at the beginning of the embryo . Common wheat The embryo extract contains RNA N- Glucosidase malt agglutinin and other translation inhibitors , Such as Thionine 、 Ribonuclease 、 Deoxyribonucleases and proteases, etc . These inhibitors are produced in the endosperm . Remove endosperm from wheat embryos by washing them thoroughly Contaminants , Form highly stable and active extracts .

Will have  5'  Terminal methylation and  3'  End  poly(A)  Tail  mRNA  React with this extract , At the same time, the unique liposome was added , The translation reaction in vitro can produce enough membrane proteins , Membrane proteins are captured by liposomes , So as to correctly fold out the protein forms necessary for the implementation of biological functions . Common protein expression systems , E. coli, for example 、 Insect cells and mammalian cells belong to the body system and are bound by the cell membrane , Its yield and stability are relatively low . Compared with these protein expression systems , AI Meijie  Abnova This system has remarkable advantages . Besides , The in vitro wheat embryo system can stand the test of automated high-throughput small molecule biological screening and characterization .

characteristic ：

Higher production

More stable

High expression success rate

Natural protein configuration

Easy to use for automation

Category ：

Cluster differentiation  (CD)  molecular

G  Protein coupled receptors  (GPCR)

ion channel

Transporters

AI Meijie  Abnova Membrane protein lipoprotein demonstration ：