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Circulating tumor cells - here comes abnova's solution
2022-07-07 07:08:00 【Sylvia_ sc】
AI Meijie Abnova Use an integrated system to enrich circulating tumor cells with reproducibility and efficiency 、 Count , High purity circulating tumor cells can be isolated . This kind of liquid biopsy is an accurate tool to identify and track the transformation and metastasis of tumors , In the new era of personalized medicine , For the diagnosis of tumor 、 prognosis 、 Treatment and monitoring , Has a significant clinical impact .
AI Meijie Abnova circulating tumor cell flow chart :
Sample demand :
Human whole blood : At least at room temperature 7.5ml The volume is collected in K2-EDTA( Purple cover ) in .
transport : During transportation, it needs to be at room temperature , After sample collection 6 Within hours .
AI Meijie Abnova circulating tumor cell Cell enrichment 、 Counting and separating (CTCatch):
Preparation of test body : Whole blood after centrifugation by density gradient .
Positive enrichment : Positive selection by surface antibodies , Enrich circulating tumor cells .
Cell count : By immunofluorescence / Fluorescence in situ hybridization (FISH), Biomarkers to identify circulating tumor cells .
Cell separation : Micromanipulation to separate circulating tumor cells , For full genome amplification (WGA) And downstream analysis .
Analysis method :
Immunofluorescence
mutaFISH
aCGH
Next generation sequencing (Abnova NGS Solution)
Abnova circulating tumor cell Report delivery (7 Within working days ):
Provide an explanation report of circulating tumor cell examination .
Antibody series for enriching circulating tumor cells
The number of circulating tumor cells identified based on biomarkers
The number of circulating tumor cells isolated for downstream analysis
Additional protein biomarkers and gene characterization required by customers
project / Content :
Test items programme 1 programme 2 programme 3 programme 4
CTC Enrichment and counting V V V V
CTC mutaFISH V
CTC Cell separation V V
CTC WGA V V
CTC Sanger sequencing V
CTC Next generation sequencing V
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