Circulating tumor cells - here comes abnova's solution

AI Meijie Abnova  Use an integrated system to enrich circulating tumor cells with reproducibility and efficiency 、 Count , High purity circulating tumor cells can be isolated . This kind of liquid biopsy is an accurate tool to identify and track the transformation and metastasis of tumors , In the new era of personalized medicine , For the diagnosis of tumor 、 prognosis 、 Treatment and monitoring , Has a significant clinical impact .

AI Meijie  Abnova  circulating tumor cell flow chart ：

Sample demand ：

Human whole blood ： At least at room temperature 7.5ml The volume is collected in K2-EDTA（ Purple cover ） in .

transport ： During transportation, it needs to be at room temperature , After sample collection 6 Within hours .

 

AI Meijie Abnova  circulating tumor cell Cell enrichment 、 Counting and separating （CTCatch）：

Preparation of test body ： Whole blood after centrifugation by density gradient .

Positive enrichment ： Positive selection by surface antibodies , Enrich circulating tumor cells .

Cell count ： By immunofluorescence / Fluorescence in situ hybridization  (FISH), Biomarkers to identify circulating tumor cells .

Cell separation ： Micromanipulation to separate circulating tumor cells , For full genome amplification （WGA） And downstream analysis .

 

Analysis method ：

Immunofluorescence

mutaFISH

aCGH

Next generation sequencing  (Abnova NGS Solution)

 

Abnova  circulating tumor cell Report delivery （7 Within working days ）：

Provide an explanation report of circulating tumor cell examination .

Antibody series for enriching circulating tumor cells

The number of circulating tumor cells identified based on biomarkers

The number of circulating tumor cells isolated for downstream analysis

Additional protein biomarkers and gene characterization required by customers

project / Content ：

Test items       programme  1      programme  2      programme  3      programme  4

CTC Enrichment and counting  V V V V

CTC mutaFISH   V    

CTC  Cell separation      V V

CTC WGA     V V

CTC Sanger  sequencing      V  

CTC  Next generation sequencing        V