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Abnova circulating tumor DNA whole blood isolation, genomic DNA extraction and analysis
2022-07-07 07:08:00 【Sylvia_ sc】
AI Meijie Abnova Have the ability to ctDNA To extract 、 purification 、 A streaming platform for library preparation and next generation sequencing .ctDNA Liquid biopsy is an identification of cancer 、 A precise tool for tracking cancer transformation and metastasis , In the era of precision medicine , This tool is useful for cancer diagnosis 、 prognosis 、 Treatment or monitoring has important clinical significance .
AI Meijie Abnova Customized circulating tumor DNA Workflow :
Sample demand :
Human whole blood : At least at room temperature 4ml Volume whole blood was collected in EDTA tube ( Purple cover ) in .
transport : During transportation, it needs to be at room temperature .
DNA The preparation of : The genome was extracted from the plasma portion of the whole blood sample on the same day of collection DNA, And in 4℃ Store... Under .
AI Meijie Abnova Whole blood separation , Genome DNA extraction , Sample library preparation , Next generation sequencing and gene mutation analysis :
DNA extraction : Extract genome from plasma layer DNA, Then proceed DNA Purification and quantification .
Library preparation : The genome DNA The fragment and size are converted into double chains DNA, Then connect the oligonucleotide adaptor , And quantify the products of the sample library .
Next generation sequencing : Sequencing of hot spots and exons in mutant genes of interest .
Gene mutation analysis : The published literatures and public databases are used to further annotate and compare cancer gene mutations and germline mutations .
Analysis method :
Next generation sequencing (Abnova NGS Solution)
Abnova circulating tumor cell Report delivery (14 Within working days ):
Provide an explanation report of cancer gene mutation .
Genes used to identify mutations in cancer genes
The gene sequence of cancer gene mutation and the gene sequence of different gene mutations obtained by next generation sequencing
Notes on cancer mutations and their comparison with known data
Additional mutation gene sequencing according to the customer's requirements
project / Content :
1、 Cancer genes
Operable gene projects related to tumors V2—— Cancer genes and mutations
ALK KIT
BRA FKRAS
EGFR NRAS
ERBB2 PDGFRA
ERBB3 PIK3CA
ESR1 RAF1
2、 Operable gene projects related to tumors V3—— Cancer genes and mutations
AKT1 GNA11
ALK GNAQ
BRAF HRAS
CTNNB1 KIT
DDR2 KRAS
EGFR MAP2K1
ERBB2 MAP2K2
ERBB3 MET
ERBB4 NOTCH1
ESR1 NRAS
FBXW7 PDGFRA
FGFR1 PIK3CA
FGFR2 RAF1
FGFR3 SMAD4
FLT3 STK11
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