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Abnova circulating tumor DNA whole blood isolation, genomic DNA extraction and analysis

2022-07-07 07:08:00 【Sylvia_ sc】

AI Meijie Abnova Have the ability to ctDNA To extract 、 purification 、 A streaming platform for library preparation and next generation sequencing .ctDNA Liquid biopsy is an identification of cancer 、 A precise tool for tracking cancer transformation and metastasis , In the era of precision medicine , This tool is useful for cancer diagnosis 、 prognosis 、 Treatment or monitoring has important clinical significance .

AI Meijie Abnova Customized circulating tumor DNA Workflow ：

Sample demand ：

Human whole blood ： At least at room temperature 4ml Volume whole blood was collected in EDTA tube （ Purple cover ） in .

transport ： During transportation, it needs to be at room temperature .

DNA The preparation of ： The genome was extracted from the plasma portion of the whole blood sample on the same day of collection DNA, And in 4℃ Store... Under .

AI Meijie Abnova Whole blood separation , Genome DNA extraction , Sample library preparation , Next generation sequencing and gene mutation analysis ：

DNA extraction ： Extract genome from plasma layer DNA, Then proceed DNA Purification and quantification .

Library preparation ： The genome DNA The fragment and size are converted into double chains DNA, Then connect the oligonucleotide adaptor , And quantify the products of the sample library .

Next generation sequencing ： Sequencing of hot spots and exons in mutant genes of interest .

Gene mutation analysis ： The published literatures and public databases are used to further annotate and compare cancer gene mutations and germline mutations .

Analysis method ：

Next generation sequencing (Abnova NGS Solution)

Abnova circulating tumor cell Report delivery （14 Within working days ）：

Provide an explanation report of cancer gene mutation .

Genes used to identify mutations in cancer genes

The gene sequence of cancer gene mutation and the gene sequence of different gene mutations obtained by next generation sequencing

Notes on cancer mutations and their comparison with known data

Additional mutation gene sequencing according to the customer's requirements

project / Content ：

1、 Cancer genes

Operable gene projects related to tumors V2—— Cancer genes and mutations

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