Abbkine trakine F-actin Staining Kit (green fluorescence) scheme

Ghost pen cyclic peptide （phalloidin）, From a highly toxic mushroom (Amanita phalloides) A polypeptide substance isolated from , It belongs to Cymbidium peptide toxin , It is a strong toxin . It is a specific binding F- Bicyclic peptide of actin , therefore , Ghost pen cyclic peptide coupled with fluorescent dye can be studied very conveniently F-actins The distribution of . Inside the ghost pen cyclopeptide , There is an unusual sulfide bridge between cysteine and tryptophan , It can form an inner ring structure . When pH When rising , Thioether is cracked , Ghost pen cyclopeptide loses its affinity for actin .

Abbkine F-actin Dye Kit （ Green fluorescence ） Component ：

 

• Ghost pen cyclic peptide  ( Green fluorescence ) (200×)

• Experimental buffer  (5×)

Abbkine F-actin Dye Kit Experimental suggestions ：

Abbkine TraKine F-actin Dye Kit （ Green fluorescence ） Green fluorescent dye is used  (Ex/Em=490/515nm)  Conjugated ghost pen cyclic peptide , Can specifically bind F-actins, And other fluorescein - Compared with ghost pen cyclopeptide derivatives , It has better light stability .

Abbkine F-actin Dye Kit characteristic ：

• Optimized experimental scheme , It is especially suitable for tissue sections fixed and permeating with formaldehyde 、 In cell culture or cell-free experiment F-actins Mark , Identification and quantitative requirements .

• Brick Li's ghost pen cyclopeptide dye  ( Green fluorescence ) (Ex/Em = 490/515 nm), Specifically bind F-actins, And it is better than other fluorescein - Ghost pen cyclopeptide conjugate has stronger photostability .

Precautions in use ：

Because the more precise staining conditions of different cell types may be different , It is suggested to determine the appropriate concentration according to the experiment .

chart ： Primary cultured mouse cardiomyocytes .