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Duchefa MS medium contains vitamin instructions
2022-07-05 20:38:00 【Sylvia_ sc】
AI Meijie Duchefa MS The medium is J Commonly used tissue culture medium , Many variants have been developed . This medium is derived from White's culture medium , It was originally developed for planting tobacco callus . Compared with white medium , The concentration of all components has increased . Increased to 50-60 mM Nitrogen significantly stimulates the growth of tobacco cells , however 80 mM And higher concentrations are obviously detrimental to cells . All other elements , Especially the increase of macro elements , It also stimulated the growth of callus . Due to the high concentration of minerals ,MS Culture medium is a kind of culture medium which is very rich in salt , It may be too salty for some plant species . To avoid this problem ,MS It is often used with trace elements in full concentration , But the macro elements are at half or three quarters of the concentration originally described by the author . Sometimes , The original MS Vitamin quilt Linsmaier and Gamborg B5 Replaced by vitamins in the culture medium , Because the concentration of thiamine is related to the demand of plants for this vitamin ..
AI Meijie Duchefa MS Detailed parameters of the medium containing vitamins :
element of a surface mg / l micron
CoCl 2 .6H 2 O 0.025 0.11
CuSO 4 .5H 2 O 0.025 0.10
FeNaEDTA 36.70 100.00
H 3 boron 3 6.20 100.27
KI 0.83 5.00
MnSO 4 .H 2 O 16.90 100.00
Na 2 MoO 4 .2H 2 O 0.25 1.03
ZnSO 4 .7H 2 O
Macro element mg / l mm
calcium chloride 2 332.02 2.99
KH 2 PO 4 170.00 1.25
Potassium nitrate 3 1900.00 18.79
magnesium sulphate 4 180.54 1.50
NH 4 NO 3 1650.00 20.61
vitamin mg / l micron
Glycine 2.00 26.64
inositol 100.00 554.94
Niacin 0.50 4.06
Pyridoxine hydrochloride 0.50 2.43
Thiamine hydrochloride 0.10 0.30
Trace elements and macro elements ( Including vitamins ) Total concentration of :4405.19 mg/l
Murashige T. and Skoog F., physiology . plant ,15,473(1962).
AI Meijie Duchefa D5124 MD5A culture medium Related research :
1、M0231
MURASHIGE & SKOOG culture medium , Include B5 vitamin
Include Gamborg B 5 vitamin
Murashige T. and Skoog F., Physiol. plant ,15,473(1962).
2、M0256
MURASHIGE & SKOOG medium , contain . Nicky vitamins
Include Nitsch vitamin
Murashige T. and Skoog F., Physiol. plant ,15,473(1962).
3、M0254
MURASHIGE & SKOOG medium , contain .MES Buffer
Include MES Buffer (500 mg/l)
Murashige T. and Skoog F., Physiol. plant ,15,473(1962).
4、M0255
MURASHIGE & SKOOG medium , contain . vitamin /MES
Include MES Buffer (500 mg/l)
Murashige T. and Skoog F., Physiol. plant ,15,473(1962).
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