Duchefa MS medium contains vitamin instructions

AI Meijie  Duchefa MS  The medium is J Commonly used tissue culture medium , Many variants have been developed . This medium is derived from White's culture medium , It was originally developed for planting tobacco callus . Compared with white medium , The concentration of all components has increased . Increased to  50-60 mM  Nitrogen significantly stimulates the growth of tobacco cells , however  80 mM  And higher concentrations are obviously detrimental to cells . All other elements , Especially the increase of macro elements , It also stimulated the growth of callus . Due to the high concentration of minerals ,MS  Culture medium is a kind of culture medium which is very rich in salt , It may be too salty for some plant species . To avoid this problem ,MS  It is often used with trace elements in full concentration , But the macro elements are at half or three quarters of the concentration originally described by the author . Sometimes , The original  MS  Vitamin quilt  Linsmaier  and  Gamborg B5  Replaced by vitamins in the culture medium , Because the concentration of thiamine is related to the demand of plants for this vitamin ..

AI Meijie  Duchefa MS Detailed parameters of the medium containing vitamins ：

element of a surface   mg / l   micron

CoCl 2 .6H 2 O 0.025 0.11

CuSO 4 .5H 2 O 0.025 0.10

FeNaEDTA 36.70 100.00

H 3 boron 3 6.20 100.27

KI 0.83 5.00

MnSO 4 .H 2 O 16.90 100.00

Na 2 MoO 4 .2H 2 O 0.25 1.03

ZnSO 4 .7H 2 O

Macro element   mg / l   mm

calcium chloride 2 332.02 2.99

KH 2 PO 4 170.00 1.25

Potassium nitrate 3 1900.00 18.79

magnesium sulphate 4 180.54 1.50

NH 4 NO 3 1650.00 20.61

vitamin   mg / l   micron

Glycine  2.00 26.64

inositol  100.00 554.94

Niacin  0.50 4.06

Pyridoxine hydrochloride  0.50 2.43

Thiamine hydrochloride  0.10 0.30

Trace elements and macro elements （ Including vitamins ） Total concentration of ：4405.19 mg/l

Murashige T.  and  Skoog F., physiology . plant ,15,473（1962）.

AI Meijie  Duchefa D5124 MD5A  culture medium Related research ：

1、M0231

MURASHIGE & SKOOG  culture medium , Include  B5  vitamin

Include  Gamborg B 5 vitamin

Murashige T.  and  Skoog F., Physiol. plant ,15,473（1962）.

2、M0256

MURASHIGE & SKOOG  medium , contain . Nicky vitamins

Include  Nitsch  vitamin

Murashige T.  and  Skoog F., Physiol. plant ,15,473（1962）.

3、M0254

MURASHIGE & SKOOG  medium , contain .MES  Buffer

Include  MES  Buffer  (500 mg/l)

Murashige T.  and  Skoog F., Physiol. plant ,15,473（1962）.

4、M0255

MURASHIGE & SKOOG  medium , contain . vitamin /MES

Include  MES  Buffer  (500 mg/l)

Murashige T.  and  Skoog F., Physiol. plant ,15,473（1962）.