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Specification of protein quantitative kit for abbkine BCA method
2022-07-05 20:39:00 【Sylvia_ sc】
Protein quantification kit (BCA Law ) It is one of the commonly used protein concentration detection methods . The principle of this method is that the protein will remove copper ions in alkaline conditions (Cu2+) Reduced to cuprous ion (Cu+), Generated Cu+ And BCA Form purple complex , And in 562 nm There is a strong absorption peak at , The absorbance value is directly proportional to the content of protein in the sample , According to the light absorption value, the protein concentration can be calculated .
Abbkine BCA Law Protein quantitative kit components :
• BCA Reagents A
• BCA Reagents B
• BSA Standards
Abbkine BCA Law Protein quantification kit Experimental suggestions :
BCA The method is suitable for testing 20-2,000 µg/mL Protein concentration in the range , The minimum amount of protein detected is 0.4 µg, The volume of the sample to be tested is 1-20 µL.
Abbkine BCA Law Protein quantification kit characteristic :
• Save time — Than traditional Lowry It's easier , Fast 4 times .
• High sensitivity — Detection limit as low as 20 ug/mL, The minimum amount of protein detected is 0.4 ug.
• Linear excellence — Linear operating range 20-2000 ug/mL.
• Good stability — Compared with the method of dye combination , The coefficient of variation of different protein molecules is smaller .
• Compatibility is good. — Not affected by most ionic and non-ionic detergents , The samples can be compatible at most 5% SDS, 5% Triton X-100, 5% Tween-20,60,80.
Precautions in use :
• 1. Determine with microplate reader 562 nm Absorbance of , Or in the 540-595 nm Absorbance between other wavelengths .
• 2. BCA The determination of protein concentration by this method is not affected by the chemicals in most samples , It can be compatible with samples up to 5% Of SDS,5% Of Triton X-100,5% Of Tween20、60、80. but BCA The method is affected by chelating agent and slightly higher concentration reducing agent , Need to ensure EDTA lower than 10 mM, nothing EGTA, Dithiothreitol (DTT) lower than 1 mM,β- Mercaptoethanol (β-Mercaptoethanol) lower than 0.01%.
• 3. It is recommended to make a standard curve for each measurement . because BCA The color will deepen as time goes on , And the color reaction will accelerate with the increase of temperature .
• 4. If the sample diluent or pyrolysis solution itself is found to have a high background , Use the Protein Quantification Kit (Bradford Law )
Abbkine BCA Law Standard curve of protein quantitative kit :
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