Specification of protein quantitative kit for abbkine BCA method

Protein quantification kit （BCA Law ） It is one of the commonly used protein concentration detection methods . The principle of this method is that the protein will remove copper ions in alkaline conditions （Cu2+） Reduced to cuprous ion （Cu+）, Generated Cu+ And BCA Form purple complex , And in 562 nm There is a strong absorption peak at , The absorbance value is directly proportional to the content of protein in the sample , According to the light absorption value, the protein concentration can be calculated .

Abbkine BCA Law Protein quantitative kit components ：

• BCA Reagents A

• BCA Reagents B

• BSA Standards

Abbkine BCA Law   Protein quantification kit Experimental suggestions ：

BCA The method is suitable for testing 20-2,000 µg/mL Protein concentration in the range , The minimum amount of protein detected is 0.4 µg, The volume of the sample to be tested is  1-20 µL.

Abbkine BCA Law   Protein quantification kit characteristic ：

• Save time — Than traditional Lowry It's easier , Fast 4 times .

• High sensitivity — Detection limit as low as 20 ug/mL, The minimum amount of protein detected is 0.4 ug.

• Linear excellence — Linear operating range 20-2000 ug/mL.

• Good stability — Compared with the method of dye combination , The coefficient of variation of different protein molecules is smaller .

• Compatibility is good. — Not affected by most ionic and non-ionic detergents , The samples can be compatible at most 5% SDS, 5% Triton X-100, 5% Tween-20,60,80.

Precautions in use ：

• 1.  Determine with microplate reader  562 nm  Absorbance of , Or in the 540-595 nm Absorbance between other wavelengths .

• 2. BCA  The determination of protein concentration by this method is not affected by the chemicals in most samples , It can be compatible with samples up to 5% Of SDS,5% Of Triton X-100,5% Of Tween20、60、80. but BCA The method is affected by chelating agent and slightly higher concentration reducing agent , Need to ensure EDTA lower than 10 mM, nothing  EGTA, Dithiothreitol (DTT) lower than 1 mM,β- Mercaptoethanol (β-Mercaptoethanol) lower than 0.01%.

• 3.  It is recommended to make a standard curve for each measurement . because BCA The color will deepen as time goes on , And the color reaction will accelerate with the increase of temperature .

• 4.  If the sample diluent or pyrolysis solution itself is found to have a high background , Use the Protein Quantification Kit （Bradford Law ）

Abbkine BCA Law   Standard curve of protein quantitative kit ：