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Abnova丨 MaxPab 小鼠源多克隆抗体解决方案
2022-07-05 20:35:00 【Sylvia_sc】
艾美捷 Abnova METTL3 纯化 MaxPab 小鼠多克隆抗体 (B01P) ——针对全长人类 METTL3 蛋白产生的小鼠多克隆抗体。
艾美捷 Abnova MaxPab 小鼠源多克隆抗体的化学性质:
免疫原:METTL3 (NP_062826.2, 1 aa ~ 580 aa) 全长人类蛋白质。
宿主:鼠
反应性:人类
种间抗原序列:鼠标 (97); 大鼠 (96)
质量控制测试:对哺乳动物转染的裂解物具有反应性的抗体。
存储缓冲区:在 1x PBS 中,pH 7.4
储存说明:储存在 -20°C 或更低。分装以避免反复冻融。
艾美捷 Abnova MaxPab 小鼠源多克隆抗体的应用:
左图:蛋白质印迹(组织裂解物)
METTL3 MaxPab 多克隆抗体。人结肠中 METTL3 表达的蛋白质印迹分析。
中图:Western Blot(转染裂解物)
通过 METTL3 MaxPab 多克隆抗体对转染的 293T 细胞系 ( H00056339-T01 )中的 METTL3 表达进行蛋白质印迹分析。
泳道 1:METTL3 转染的裂解物(63.8 KDa)。
泳道 2:未转染的裂解物。
右图:免疫荧光
HeLa 细胞上针对 METTL3的纯化MaxPab 抗体的免疫荧光。[抗体浓度10 ug/ml]
Abnova MaxPab 小鼠源多克隆抗体部分文献参考:
1.Relaxed initiation pausing of ribosomes drives oncogenic translation.
Leiming Dong, Yuanhui Mao, Aidong Zhou, Xiao-Min Liu, Jun Zhou, Ji Wan, Shu-Bing Qian.Sci Adv. 2021 Feb 17;7(8):eabd6927. doi: 10.1126/sciadv.abd6927. Print 2021 Feb.
2.m6A in mRNA coding regions promotes translation via the RNA helicase-containing YTHDC2.
Mao Y, Dong L, Liu XM, Guo J, Ma H, Shen B, Qian SB.Nat Commun. 2019 Nov 25;10(1):5332. doi: 10.1038/s41467-019-13317-9.
3.Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication.
Tsai K, Courtney DG, Cullen BR.PLoS Pathog. 2018 Feb 15;14(2):e1006919. doi: 10.1371/journal.ppat.1006919. eCollection 2018 Feb.
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