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Abnova 膜蛋白脂蛋白体技术及类别展示
2022-07-07 02:11:00 【Sylvia_sc】
体外小麦胚系统 + 脂蛋白体技术
艾美捷 Abnova体外蛋白质表达系统以小麦胚真核翻译机制为基础开发而成。小麦胚将所有翻译所需的成分存储于干燥浓缩的状态,在胚芽开始时即进行蛋白质的合成。普通的小麦 胚萃取物中包含有RNA N-糖苷酶麦芽凝集素和其它翻译抑制物,例如硫堇、核糖核酸酶、脱氧核糖核酸酶类和蛋白酶等。这类抑制物产生于胚乳中。通过充分清洗小麦胚去除其中的胚乳 污染物,形成高稳定性和高活性的萃取物。
将具有 5' 端甲基化与 3' 端 poly(A) 尾的 mRNA 与这种萃取物反应,同时加入独门脂质体,体外的翻译反应能产生出足量的膜蛋白,膜蛋白被脂质体捕获,从而正确折迭出执行生物功能所必需的蛋白质形态。常用的蛋白质表达系统,例如大肠杆菌、昆虫细胞和哺乳动物细胞等都属于体内系统并且受到细胞膜的约束作用,其产量和稳定性都相对较低。与这些蛋白质表达系统相比,艾美捷 Abnova这一系统有着显着的优越性。此外,体外小麦胚系统能够经得起自动化高通量小分子生物筛选以及特征描述的考验。
特点:
产量更高
稳定性更高
高表达成功率
天然蛋白构型
易用于自动化
类别:
聚类分化 (CD) 分子
G 蛋白偶联受体 (GPCR)
离子通道
转运蛋白
艾美捷 Abnova膜蛋白脂蛋白体演示:
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