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Norgen AAV extractant box instructions (including features)
2022-07-05 20:39:00 【Sylvia_ sc】
AI Meijie Norgen AAV Extractant box background :
Recombinant adeno-associated virus (AAV) Vector is a very promising tool for gene transfer in vitro and in vivo .Norgen Of AAV The purification kit is concentrated and purified from cell lysate and cell culture medium AAV Carrier provides a fast and simple method . Purification is based on use Norgen Rotating chromatographic column with proprietary resin as ion exchanger . Most of the contaminated cell fragments are removed from the sample by precipitation and centrifugation , At the same time, enzyme digestion and column purification are used to reduce pollution DNA and RNA. Purified in this way AAV The vector has high activity in vitro transduction experiments , And elute into a small volume using an optional concentration step (200 µL).
AI Meijie Norgen AAV Chemical properties of extractant box :
full name :AAV Purification kit ( Extraction Kit )
english :AAV Purification Mini Kit
cat#:NGB-63200
specifications :20Preps
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DNAse I and RNAse A Stored in the -20°C. The elution buffer is sealed and stored in 4°C. All other solutions should be sealed and stored at room temperature . Once opened , The solution should be stored in 4°C. All reagents should be kept stable in unopened containers for at least 1 year . Please store according to the instructions for specific storage .
AI Meijie Norgen AAV Characteristics of extractant box :
From any input AAV purification —— Cellular part 、 Medium part or mixture of cells and medium
1 to 2 Rapid purification within hours
high AAV Recovery rate , the height is 90%
No special equipment is required
From a variety of AAV Purified from serotype
from 0.5 mL To 8 mL Input purification AAV
Produce high activity for in vitro experiments AAV
the height is 50 Times the sample concentration
It can be purified many times at the same time ( Each kit 20 Time ) To conduct a rapid screening experiment
Purification is based on use Norgen Proprietary silicon carbide resin separation matrix rotating column chromatography
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