Norgen AAV extractant box instructions (including features)

AI Meijie  Norgen AAV Extractant box background ：

Recombinant adeno-associated virus （AAV） Vector is a very promising tool for gene transfer in vitro and in vivo .Norgen Of AAV The purification kit is concentrated and purified from cell lysate and cell culture medium AAV Carrier provides a fast and simple method . Purification is based on use Norgen Rotating chromatographic column with proprietary resin as ion exchanger . Most of the contaminated cell fragments are removed from the sample by precipitation and centrifugation , At the same time, enzyme digestion and column purification are used to reduce pollution DNA and RNA. Purified in this way AAV The vector has high activity in vitro transduction experiments , And elute into a small volume using an optional concentration step （200 µL）.

AI Meijie  Norgen AAV Chemical properties of extractant box ：

full name ：AAV Purification kit （ Extraction Kit ）

english ：AAV Purification Mini Kit

cat#：NGB-63200

specifications ：20Preps

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DNAse I and RNAse A Stored in the -20°C. The elution buffer is sealed and stored in 4°C. All other solutions should be sealed and stored at room temperature . Once opened , The solution should be stored in 4°C. All reagents should be kept stable in unopened containers for at least 1 year . Please store according to the instructions for specific storage .

AI Meijie  Norgen AAV Characteristics of extractant box ：

From any input  AAV  purification —— Cellular part 、 Medium part or mixture of cells and medium

1 to 2 Rapid purification within hours

high  AAV  Recovery rate , the height is  90%

No special equipment is required

From a variety of  AAV  Purified from serotype

from  0.5 mL  To  8 mL  Input purification  AAV

Produce high activity for in vitro experiments  AAV

the height is  50  Times the sample concentration

It can be purified many times at the same time （ Each kit  20  Time ） To conduct a rapid screening experiment

Purification is based on use  Norgen  Proprietary silicon carbide resin separation matrix rotating column chromatography