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Chemical properties and application instructions of prosci Lag3 antibody
2022-07-05 20:39:00 【Sylvia_ sc】
Lymphocyte activating gene 3 (LAG3) Is a member of the immunoglobulin superfamily , Combine with high affinity MHC II class , Negative regulation T Cell function and homeostasis . It's in B、T and NK cells 、 It is expressed in monocytes and dendritic cells , And play a regulating role T The role of cell expansion .LAG3 It is also an important immune checkpoint protein , resist LAG3 Antibodies can activate T Effector cells and affect regulatory T Cell function . Besides ,LAG3 It seems to be with PD-1/PD-L1 Work in a collaborative way , This indicates that the double antibody method may prove useful in cancer immunotherapy .
AI Meijie ProSci LAG3 Chemical properties of antibodies :
Host species : rat
Species reactivity : human beings
Immunogen :LAG-3 Antibodies are directed against people LAG-3 Produced by the extracellular domain of
The test application :ICC、IF、IHC-P、WB etc.
purification :LAG-3 Antibodies are expressed in proteins A purification IgG1 The form provides .
Clonality : Monoclonal
Same type :IgG1,k
Physical state : Liquid
buffer :LAG-3 Antibodies contain 0.02% Sodium azide and 50% Glycerine PBS The form provides .
Storage conditions :LAG-3 Antibodies can be found in 4°C and -20°C Keep it for three months , Stable for a year . Like all antibodies , Care should be taken to avoid repeated freeze-thaw cycles . Antibodies should not be exposed to high temperature for a long time .
AI Meijie ProSci LAG3 Antibody application instructions :
Can be used for ELISA, The initial concentration is 0.25 μg/mL. For Western blotting , The initial concentration is 0.25 μg/mL. For immunocytochemistry , from 1 μg/mL Start . For immunofluorescence , from 10 μg/mL Start . For immunohistochemistry , from 5 μg/mL Start . For flow cytometry , from 1 μg/ml Start .
AI Meijie ProSci LAG3 Antibody validation :
Western blotting in human samples ; Immunohistochemistry in human samples ; Immunocytochemistry in human samples ; Immunofluorescence in human samples and flow cytometry in human samples . All other applications and species have not been tested .
Positive control 1: cat .No. 1331 - Human lymphoma tissue lysate
Positive control 2: cat .No. 1306 - Human spleen tissue
Estimated molecular weight : forecast :55 kDa
The observed :60、70、80 kDa
chart : Use (A) 0.25 (B) 0.5 and (C) 1 μg/ml Of LAG-3 Antibody pair overexpression HEK293 In cells LAG-3 Western blot analysis was performed .
LAG3 antibody Citations :
1:Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.
2:Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.
3:Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.
4:Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.
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