Chemical properties and application instructions of prosci Lag3 antibody

Lymphocyte activating gene  3 (LAG3)  Is a member of the immunoglobulin superfamily , Combine with high affinity  MHC II  class , Negative regulation  T  Cell function and homeostasis . It's in  B、T  and  NK  cells 、 It is expressed in monocytes and dendritic cells , And play a regulating role  T  The role of cell expansion .LAG3  It is also an important immune checkpoint protein , resist  LAG3  Antibodies can activate  T  Effector cells and affect regulatory  T  Cell function . Besides ,LAG3  It seems to be with  PD-1/PD-L1  Work in a collaborative way , This indicates that the double antibody method may prove useful in cancer immunotherapy .

AI Meijie ProSci LAG3 Chemical properties of antibodies ：

Host species ： rat

Species reactivity ： human beings

Immunogen ：LAG-3  Antibodies are directed against people  LAG-3  Produced by the extracellular domain of

The test application ：ICC、IF、IHC-P、WB etc.

purification ：LAG-3  Antibodies are expressed in proteins  A  purification  IgG1  The form provides .

Clonality ： Monoclonal

Same type ：IgG1,k

Physical state ： Liquid

buffer ：LAG-3  Antibodies contain  0.02%  Sodium azide and  50%  Glycerine  PBS  The form provides .

Storage conditions ：LAG-3  Antibodies can be found in  4°C  and  -20°C  Keep it for three months , Stable for a year . Like all antibodies , Care should be taken to avoid repeated freeze-thaw cycles . Antibodies should not be exposed to high temperature for a long time .

AI Meijie ProSci LAG3 Antibody application instructions ：

Can be used for  ELISA, The initial concentration is  0.25 μg/mL. For Western blotting , The initial concentration is  0.25 μg/mL. For immunocytochemistry , from  1 μg/mL  Start . For immunofluorescence , from  10 μg/mL  Start . For immunohistochemistry , from  5 μg/mL  Start . For flow cytometry , from  1 μg/ml  Start .

AI Meijie ProSci LAG3 Antibody validation ：

Western blotting in human samples ; Immunohistochemistry in human samples ; Immunocytochemistry in human samples ; Immunofluorescence in human samples and flow cytometry in human samples . All other applications and species have not been tested .

Positive control 1： cat .No. 1331 -  Human lymphoma tissue lysate

Positive control 2： cat .No. 1306 -  Human spleen tissue

Estimated molecular weight ： forecast ：55 kDa

The observed ：60、70、80 kDa

chart ： Use  (A) 0.25 (B) 0.5  and  (C) 1 μg/ml  Of  LAG-3  Antibody pair overexpression  HEK293  In cells  LAG-3  Western blot analysis was performed .

LAG3 antibody Citations ：

1:Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.

2:Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.

3:Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.

4:Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.