Introduction to abnova's in vitro mRNA transcription workflow and capping method

In vitro transcription （IVT）mRNA It is widely used in basic research and Application , It provides a promising gene expression system for rapid and controllable induction of target proteins . Based on its security and efficiency ,IVT mRNA Is a powerful method , In regenerative medicine 、 vaccination 、 There are potential applications in cell engineering and protein replacement therapy . With internal epigenetic modification IVT RNA Synthesis requires DNA Templates 、 enzyme 、 Nucleotides and buffer ingredients . Not familiar with IVT mRNA Technology or looking for fast and effective services ？

AI Meijie  Abnova There are abundant IVT mRNA service , Include DNA Template production 、mRNA Production and mRNA The quality control .

AI Meijie Abnova IVT mRNA Workflow ：

Customization from hundreds of bases to thousands of bases mRNA

Optimization of the mRNA Synthesis and purification procedures

Cell free production , No toxic chemicals

Eligible RNA To meet customer needs

It can be used in most downstream applications

AI Meijie  Abnova IVT synthesis mRNA Service details ：

1、DNA Production of formwork

Amplify genes of interest * And clone it into the expression vector

plasmid DNA Preparation and purification of

Linearized DNA The preparation of

2、mRNA production

Linearized DNA The preparation of

Linearization DNA In vitro transcribed into RNA

RNA Cap

Capped RNA Purification and quantification **

3、mRNA The quality control

Integrity and purity ：RNA Agarose gel electrophoresis

Protein expression : RNA Transfection into cells , Cell lysate Western blot analysis .

 

Adding method ：

Co transcriptional modification

Sequence  RNA  Sequence   Capping sequence   Hat analogues

Cap 0 GAUG 3´-O-Me-m7G(5')ppp(5')GAUG Anti-Reverse

GTP competition

Cap 1 AUG m7G(5')ppp(5')(2'OMeA)pUG Without Anti-Reverse

Without GTP competition

Cap 1 AGG m7(3'OMeG)(5')ppp(5')(2'OMeG)pGG Anti-Reverse

Without GTP competition

Cap 1 GGG m7(3'OMeG)(5')ppp(5')(2'OMeA)pGG Anti-Reverse

GTP competition

 

Nucleoside modification ：

Pseudouridine (Ψ)

5-methylcytidine (m5C)

N1-methylpseudouridine (N1mΨ)