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Introduction to abnova's in vitro mRNA transcription workflow and capping method
2022-07-07 07:10:00 【Sylvia_ sc】
In vitro transcription (IVT)mRNA It is widely used in basic research and Application , It provides a promising gene expression system for rapid and controllable induction of target proteins . Based on its security and efficiency ,IVT mRNA Is a powerful method , In regenerative medicine 、 vaccination 、 There are potential applications in cell engineering and protein replacement therapy . With internal epigenetic modification IVT RNA Synthesis requires DNA Templates 、 enzyme 、 Nucleotides and buffer ingredients . Not familiar with IVT mRNA Technology or looking for fast and effective services ?
AI Meijie Abnova There are abundant IVT mRNA service , Include DNA Template production 、mRNA Production and mRNA The quality control .
AI Meijie Abnova IVT mRNA Workflow :
Customization from hundreds of bases to thousands of bases mRNA
Optimization of the mRNA Synthesis and purification procedures
Cell free production , No toxic chemicals
Eligible RNA To meet customer needs
It can be used in most downstream applications
AI Meijie Abnova IVT synthesis mRNA Service details :
1、DNA Production of formwork
Amplify genes of interest * And clone it into the expression vector
plasmid DNA Preparation and purification of
Linearized DNA The preparation of
2、mRNA production
Linearized DNA The preparation of
Linearization DNA In vitro transcribed into RNA
RNA Cap
Capped RNA Purification and quantification **
3、mRNA The quality control
Integrity and purity :RNA Agarose gel electrophoresis
Protein expression : RNA Transfection into cells , Cell lysate Western blot analysis .
Adding method :
Co transcriptional modification
Sequence RNA Sequence Capping sequence Hat analogues
Cap 0 GAUG 3´-O-Me-m7G(5')ppp(5')GAUG Anti-Reverse
GTP competition
Cap 1 AUG m7G(5')ppp(5')(2'OMeA)pUG Without Anti-Reverse
Without GTP competition
Cap 1 AGG m7(3'OMeG)(5')ppp(5')(2'OMeG)pGG Anti-Reverse
Without GTP competition
Cap 1 GGG m7(3'OMeG)(5')ppp(5')(2'OMeA)pGG Anti-Reverse
GTP competition
Nucleoside modification :
Pseudouridine (Ψ)
5-methylcytidine (m5C)
N1-methylpseudouridine (N1mΨ)
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