Fast quantitative, abbkine protein quantitative kit BCA method is coming!

In the extraction of protein 、 During purification and analysis , The quantification of protein can be seen everywhere . For example, after splitting cells , We quantify the protein in each sample , Facilitate parallel comparison in downstream applications ; Before protein electrophoresis , We also need accurate quantitative , Avoid carefully prepared electrophoresis （ especially 2D electrophoresis ） Come back with regret . Given that there may be a variety of （ interfere ） Component , There are also many methods to determine protein concentration .

BCA Method is a commonly used protein quantitative method . at present Google Academic search has 14 It turned out to be a million .BCA The basic principle of law is , Proteins interact with Cu2+ Complexation , And will Cu2+ Revert to Cu1+. Through two BCA Molecules and reduced states Cu1+ Chelation of , It can form purple color reaction products . The product is in 562 nm There is a high light absorption value at , And is directly proportional to the protein concentration .

Besides ,BCA Law has a high degree of uniformity . be relative to Bradford In terms of dye binding methods , There is less variability between different proteins .

The next thing we want to say is Abbkine Of BCA Method protein quantitative kit .

Abbkine BCA Law   Protein quantification kit Experimental suggestions ：

BCA The method is suitable for testing 20-2,000 µg/mL Protein concentration in the range , The minimum amount of protein detected is 0.4 µg, The volume of the sample to be tested is  1-20 µL.

Abbkine BCA Law   Protein quantification kit characteristic ：

• Save time — Than traditional Lowry It's easier , Fast 4 times .

• High sensitivity — Detection limit as low as 20 ug/mL, The minimum amount of protein detected is 0.4 ug.

• Linear excellence — Linear operating range 20-2000 ug/mL.

• Good stability — Compared with the method of dye combination , The coefficient of variation of different protein molecules is smaller .

• Compatibility is good. — Not affected by most ionic and non-ionic detergents , The samples can be compatible at most 5% SDS, 5% Triton X-100, 5% Tween-20,60,80.

Abbkine BCA Law   Standard curve of protein quantitative kit ：