Duchefa d5124 md5a medium Chinese and English instructions

AI Meijie  Duchefa mD5A（ Improved  D-5  Agar medium ） Used to detect seed transmission  Xanthomonas campestris pv.carota (Xccar), The pathogen of carrot bacterial wilt . Contaminated seed batches can be passed on  mD5A  Dilute and inoculate bacterial extracts with another semi selective medium to detect . Then transfer the suspected isolate to  YDC. Last , Can pass  PCR  Identify the suspected isolate .mD5A  On the medium  Xccar  The colonies are straw yellow 、 Twinkle 、 circular 、 smooth 、 bulge , The diameter is  2-3  mm .

AI Meijie  Duchefa D5124 MD5A  Medium details ：

AI Meijie  Duchefa D5124 MD5A  culture medium Related research ：

C1715

CZAPEK DOX  agar ,CDA

Czapex Dox Agar  The culture medium is used to cultivate fungi and bacteria that can use sodium nitrate as a nitrogen source .

C5140

CKTM culture medium

CKTM The medium is a semi selective medium , And improved TMB culture medium （T5126） or MXV culture medium （M5131） In combination with , Can be detected Xanthomonas campestris pv. Pepper and tomato seeds  vesicatoria (Xcv). contain  CKTM  On the plate of the medium  Xcv  The colonies are yellow 、 Mucinous 、 Mound and round .

D5128

D2ANX  culture medium

D2ANX  It is a semi selective medium , Used to detect Clavularia subspecies in Michigan . Michigan （ centimeter ）. This relatively low medium is usually associated with a more selective  mSCM  culture medium  (S5127)  Use a combination of . Even though  Cmm  The colony grows slowly , But incubating  6-7  The tablet can be evaluated after days . stay  mSCM  On , Grow more slowly ,Cmm  The colony can only be about  9-10  Days later, I can see . stay  D2ANX  On ,Cmm  The colonies glitter , Yellow and mucilaginous .

D5124

MD5A MEDIUM

Description：

mD5A (modified D-5 Agar medium) is used to detect seed borne Xanthomonas campestris pv. carota (Xccar), the causal organism of bacterial blight of carrots. Contaminated seed lots can be detected by dilution plating of the bacterial extract on mD5A and another semi-selective medium. Suspected isolates are then transferred to YDC. Finally, the identity of the suspected isolates can be determined by PCR. Colonies of Xccar on mD5A medium look straw-yellow, glistening, round, smooth, convex and are 2–3 mm in diameter. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.