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Duchefa d5124 md5a medium Chinese and English instructions
2022-07-05 20:38:00 【Sylvia_ sc】
AI Meijie Duchefa mD5A( Improved D-5 Agar medium ) Used to detect seed transmission Xanthomonas campestris pv.carota (Xccar), The pathogen of carrot bacterial wilt . Contaminated seed batches can be passed on mD5A Dilute and inoculate bacterial extracts with another semi selective medium to detect . Then transfer the suspected isolate to YDC. Last , Can pass PCR Identify the suspected isolate .mD5A On the medium Xccar The colonies are straw yellow 、 Twinkle 、 circular 、 smooth 、 bulge , The diameter is 2-3 mm .
AI Meijie Duchefa D5124 MD5A Medium details :
AI Meijie Duchefa D5124 MD5A culture medium Related research :
C1715
CZAPEK DOX agar ,CDA
Czapex Dox Agar The culture medium is used to cultivate fungi and bacteria that can use sodium nitrate as a nitrogen source .
C5140
CKTM culture medium
CKTM The medium is a semi selective medium , And improved TMB culture medium (T5126) or MXV culture medium (M5131) In combination with , Can be detected Xanthomonas campestris pv. Pepper and tomato seeds vesicatoria (Xcv). contain CKTM On the plate of the medium Xcv The colonies are yellow 、 Mucinous 、 Mound and round .
D5128
D2ANX culture medium
D2ANX It is a semi selective medium , Used to detect Clavularia subspecies in Michigan . Michigan ( centimeter ). This relatively low medium is usually associated with a more selective mSCM culture medium (S5127) Use a combination of . Even though Cmm The colony grows slowly , But incubating 6-7 The tablet can be evaluated after days . stay mSCM On , Grow more slowly ,Cmm The colony can only be about 9-10 Days later, I can see . stay D2ANX On ,Cmm The colonies glitter , Yellow and mucilaginous .
D5124
MD5A MEDIUM
Description:
mD5A (modified D-5 Agar medium) is used to detect seed borne Xanthomonas campestris pv. carota (Xccar), the causal organism of bacterial blight of carrots. Contaminated seed lots can be detected by dilution plating of the bacterial extract on mD5A and another semi-selective medium. Suspected isolates are then transferred to YDC. Finally, the identity of the suspected isolates can be determined by PCR. Colonies of Xccar on mD5A medium look straw-yellow, glistening, round, smooth, convex and are 2–3 mm in diameter. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.
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